Performance Data
These data show the TC20 automated cell counter is compatible with a broad range of cell types, such as cell lines, primary cells (from tissue or blood), and stem cells. The TC20 cell counter can count cells with a 6–50 µm cell diameter and within a broad concentration range of
5 x 104–1 x 107 cells/ml. This eliminates the need to dilute cells, thus reducing the error associated with sample dilutions prior to counting.
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The TC20 cell counter demonstrates accurate cell counts across a range of cell sizes. Small (PBMC, Jurkat), medium (HeLa), and large (MEF) cells were counted with a hemocytometer, a TC20 automated cell counter, and a competitor’s image-based automated cell counter. The TC20 counter and hemocytometer cell counts showed no statistically significant differences. Precision is indicated by the standard deviations; error bars represent average standard deviations. Cell counts on the TC20 counter were performed on one instrument with four sample replicates.
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The TC20 cell counter demonstrates accurate counts of viable cells. Pan T cells mixed with trypan blue (1:1) were counted with a hemocytometer, a TC20 automated cell counter, and a competitor’s image-based automated cell counter. The TC20 counter and hemocytometer cell counts showed no statistically significant differences. Precision is indicated by the standard deviations; error bars represent average standard deviations. Cell counts on the TC20 counter were performed on four different instruments with five sample replicates.