Performance Data

These data show the TC20 automated cell counter is compatible with a broad range of cell types, such as cell lines, primary cells (from tissue or blood), and stem cells. The TC20 cell counter can count cells with a 6–50 µm cell diameter and within a broad concentration range of
5 x 104–1 x 107 cells/ml. This eliminates the need to dilute cells, thus reducing the error associated with sample dilutions prior to counting.

TC20™ Automated Cell Counter
Cell Count Range

The TC20 cell counter demonstrates accurate cell counts across a range of cell sizes. Small (PBMC, Jurkat), medium (HeLa), and large (MEF) cells were counted with a hemocytometer, a TC20 automated cell counter, and a competitor’s image-based automated cell counter. The TC20 counter and hemocytometer cell counts showed no statistically significant differences. Precision is indicated by the standard deviations; error bars represent average standard deviations. Cell counts on the TC20 counter were performed on one instrument with four sample replicates.

Cell Count Viability

The TC20 cell counter demonstrates accurate counts of viable cells. Pan T cells mixed with trypan blue (1:1) were counted with a hemocytometer, a TC20 automated cell counter, and a competitor’s image-based automated cell counter. The TC20 counter and hemocytometer cell counts showed no statistically significant differences. Precision is indicated by the standard deviations; error bars represent average standard deviations. Cell counts on the TC20 counter were performed on four different instruments with five sample replicates.