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ROBUST SYSTEM PUMPS TO SEPARATE BIOMOLECULES FROM AQUEOUS AND ORGANIC SAMPLES

The system pump capabilities can be extended

  • Pumps are constructed of PEEK polymer, the same material used in chromatography tubing
  • Incorporate buffer inlets for multiple buffer selection
  • Add a buffer-blending module for automated method scouting

SAMPLE LOADING SOLUTIONS SPANNING LARGE VOLUME RANGES AND THROUGHPUT REQUIREMENTS

  • Use standard fixed-size sample loops and dynamic loops
  • Perform automated, accurate, and reproducible multiple injections (5 µl–5 ml) using an autosampler
  • Use a sample loading pump (flow rates of up to 100 ml/min) and an air sensor for:
– Loading of large sample volumes directly to the column
– Extending the life span of system pumps

VARIOUS COLUMN CONFIGURATIONS FOR COLUMN SCOUTING AND REDUCED NEED TO RE-PLUMB

  • Add a single column switching module to connect up to five columns
  • Add multiple column switching valves
– For multi-dimensional purification
– For reverse flow capability, which is ideal for column cleaning

UV MODULES THAT COME STANDARD WITH CONDUCTIVITY MONITORS PROVIDE OPTIMAL RESULTS

  • The single-wavelength detector module utilizes an innovative LED light source that offers extended lamp life
  • The multi-wavelength detector module can monitor up to four wavelengths simultaneously between 190–800 nm

MULTIPLE SAMPLE COLLECTION OPTIONS TO COLLECT EVERY DROP OF YOUR PRECIOUS PURIFIED SAMPLE

  • Use the BioFrac™ fraction collector — choose from rack options such as 96-well microtiter plates, standard 12 mm tubes, 15 and 50 ml conical tubes
  • Add one or more 12-port outlet modules to collect your sample into any other types of vessel

DUAL-GRADIENT PUMP SYSTEM

ACCURATE GRADIENTS FOR HIGH-RESOLUTION SEPARATIONS FOR ANY APPLICATION

Includes two gradient pumps and a mixer module to create precise gradients from two buffers.

  • Available in 0.001–10 ml/min or 0.01–100 ml/min formats
  • Up to 3,650 psi and 1,450 psi for the 10 ml/min and 100 ml/min pumps, respectively
  • Used with a wide range of media, from soft, large bead media to small, rigid media

DUAL-GRADIENT PUMP SYSTEM WITH BLENDING QUARTENARY VALVE

AUTOMATED GRADIENTS AND BLENDING FOR RAPID SCOUTING AND METHOD DEVELOPMENT

Includes two gradient pumps, a mixer module, and a quaternary valve for buffer-blending and scouting applications.

  • Available in 0.001–10 ml/min (max. 3,650 psi) and 0.01–100 ml/min (max. 1,450 psi) formats
  • Automated gradient and buffer-blending capabilities
  • Gradient separations at different pH values for rapid method development
  • Flow rates of up to 20 ml/min and 200 ml/min for the 10 ml/min and 100 ml/min pumps, respectively

DUAL-GRADIENT PUMP SYSTEM WITH BLENDING QUARTENARY VALVE AND TWO 8-PORT BUFFER INLETS

AUTOMATED BUFFER BLENDING COMBINED WITH AUTOMATED GRADIENTS USING A WIDE VARIETY OF BUFFERS FOR RAPID EXPANDED SCOUTING

Includes two gradient pumps, a mixer module, two inlet valves for automated buffer selection, and a quaternary valve for buffer-blending and scouting applications.

  • Available in 0.001–10 ml/min (max. 3,650 psi) and 0.01–100 ml/min (max. 1,450 psi) formats
  • Automated buffer gradient and buffer-blending capabilities
  • Buffer screening and gradient separations at different pH values
  • Additional inlets for buffer, cleaning, and storage solutions for fully automated workflows and walk-away convenience
  • Buffer blending valve enables flow rates of up to 20 ml/min and 200 ml/min for the 10 ml/min and 100 ml/min pumps, respectively

DUAL-GRADIENT PUMP SYSTEM WITH TWO 8-PORT BUFFER INLETS

AUTOMATED BUFFER SELECTION AND SCOUTING

Includes two gradient pumps, a mixer module, and two inlet valves.

  • Available in 0.001–10 ml/min (max. 3,650 psi) and 0.01–100 ml/min (max. 1,450 psi) formats
  • Additional inlets for buffer and cleaning, and storage solutions for fully automated workflows and walk-away convenience

DUAL-GRADIENT PUMP SYSTEM WITH AN 8-PORT BUFFER INLET

AUTOMATED BUFFER SELECTION AND SCOUTING

Includes two gradient pumps, a mixer module, and a single inlet valve

  • Available in 0.001–10 ml/min (max. 3,650 psi) and 0.01–100 ml/min (max. 1,450 psi) formats
  • Additional inlets for buffer and cleaning, and storage solutions for fully automated workflows and walk-away convenience

MANUAL SAMPLE LOADING

SMALL SAMPLE VOLUME INJECTIONS USING FIXED OR DYNAMIC LOOPS

Includes an 8-port inject valve and a fixed sample loop.

  • Highly reproducible sample injections
  • Sample injection integrated into method
  • Multiple injections of different volumes using a dynamic loop

AUTOMATED SAMPLE LOADING

LARGE SAMPLE VOLUME INJECTIONS AND AUTOMATED REPEAT LOADING OF SAMPLES

Includes a sample pump with a pressure sensor.

  • Dedicated pump for loading sample, eliminating contamination of system pump
  • Automated loading of large volume samples either directly or via sample loops
  • Flow rate of up to 100 ml/min at 1,450 psi

AUTOSAMPLER

AUTOMATED INJECTION OF A WIDE VARIETY OF SAMPLES

  • The NGC system can connect to third-party autosamplers through the signal import module (SIM)
  • We recommend the Teledyne CETAC ASX-560 autosampler for large-volume samples and the Spark Holland Alias autosampler for small-volume samples

AUTOMATED SAMPLE LOADING WITH ONE 8-PORT SAMPLE INLET

LARGE SAMPLE VOLUME INJECTIONS AND AUTOMATED REPEAT LOADING OF SAMPLES

Includes an 8-port inject valve, a sample pump, a sample inlet valve, and a fixed loop.

  • Dedicated pump for loading sample, eliminating contamination of system pump
  • Sequential automated purification of up to 8 large volume samples
  • Automated sample selection of up to 8 samples by inlet valve
  • Flow rate of up to 100 ml/min at 1,450 psi

AUTOMATED SAMPLE LOADING WITH TWO 8-PORT SAMPLE INLETS

LARGE SAMPLE VOLUME INJECTIONS AND AUTOMATED REPEAT LOADING OF SAMPLES

Includes an 8-port inject valve, a sample pump, two sample inlet valves, and a fixed loop.

  • Dedicated pump for loading sample, eliminating contamination of system pump
  • Sequential automated purification of up to 15 large volume samples
  • Flow rate of up to 100 ml/min at 1,450 psi
  • Automated sample selection of up to 15 samples by two sample inlet valves

SINGLE COLUMN

CONNECTION TO ANY COLUMN AND MEDIA TYPE

For use with most commercially available self-packed, prepacked, glass, or stainless steel lab-scale columns or cartridges.

5-COLUMN SCOUTING / SCREENING

CONNECTION OF UP TO FIVE COLUMNS

Accommodates up to five columns with reverse flow capability for increased flexibility in column and media selection.

  • Rapid screening of up to 5 columns and media in automated sequence
  • Reverse flow capability for concentrated fractions and column cleaning
  • Built-in pressure sensors protect columns from overpressurizing and compacting
  • Differential pressure sensing capability for monitoring column health

2 x 5-COLUMN SCOUTING / SCREENING

CONNECTING UP TO TEN COLUMNS AND PERFORMING TANDEM AND 2-D PURIFICATIONS

Accommodates up to ten columns with reverse flow capability for increased flexibility in column and media selection.

  • Rapid screening of up to 10 columns and media in automated sequence
  • Reverse flow capability for concentrated fractions and column cleaning
  • Built-in pressure sensors protect columns from overpressurizing and compacting
  • Differential pressure sensing capability for monitoring column health
  • Can be used as a loop valve in multicolumn (2-D) purification schemes

3 x 5-COLUMN SCOUTING / SCREENING

CONNECTING UP TO 15 COLUMNS AND PERFORMING TANDEM AND 3-D PURIFICATIONS

Accommodates up to 15 columns with reverse flow capability for increased flexibility in column and media selection.

  • Rapid screening of up to 15 columns and media in automated sequence
  • Reverse flow capability for concentrated fractions and column cleaning
  • Built-in pressure sensors protect columns from overpressurizing and compacting
  • Differential pressure sensing capability for monitoring column health
  • Can be used as a loop valve in multicolumn (3-D) purification schemes

SINGLE-WAVELENGTH DETECTOR

LED-BASED SINGLE LIGHT SOURCE DETECTION WITH CONDUCTIVITY MONITORING

Includes a high-accuracy single-wavelength detector with integrated conductivity meter.

  • Long-life LED-based light source (255 and 280 nm)
  • Integrated conductivity detector (0.01–999 mS/cm)
  • Use signal import module (SIM) to connect third-party detectors for increased characterization and autosamplers for repeat automated sample loading

MULTI-WAVELENGTH DETECTOR

SIMULTANEOUS FOUR-WAVELENGTH DETECTION WITH CONDUCTIVITY MEASUREMENT

Includes a four-wavelength detector with integrated conductivity meter.

  • 4 user-defined wavelengths (190–800 nm)
  • Integrated conductivity detector (0.01–999 mS/cm)
  • Detection of peptides, proteins, nucleic acids, and specific chromophores such as hemoglobin
  • Use signal import module (SIM) to connect third-party detectors for increased characterization and autosamplers for repeat automated sample loading

SINGLE-WAVELENGTH DETECTOR WITH pH MONITOR

LED-BASED SINGLE LIGHT SOURCE DETECTION WITH CONDUCTIVITY AND pH MONITORING

Includes a high-accuracy single-wavelength detector with integrated conductivity and pH detector and a valve.

  • Long-life LED-based light source (255 and 280 nm)
  • Integrated conductivity detector (0.01–999 mS/cm)
  • Full-range pH detection, includes pH probe and valve-based flow cell for convenient bypass, calibration, and storage
  • Use signal import module (SIM) to connect third-party detectors for increased characterization and autosamplers for repeat automated sample loading

MULTI-WAVELENGTH DETECTOR WITH pH MONITOR

SIMULTANEOUS FOUR-WAVELENGTH DETECTION WITH CONDUCTIVITY AND pH MONITORING

Includes a four-wavelength detector with integrated conductivity and pH detector and a valve.

  • 4 user-defined wavelengths (190–800 nm)
  • Detection of peptides, proteins, nucleic acids, and specific chromophores such as hemoglobin
  • Integrated conductivity detector (0.01–999 mS/cm)
  • Full-range pH detection, includes pH probe and valve-based flow cell for convenient bypass, calibration, and storage
  • Use signal import module (SIM) to connect third-party detectors for increased characterization and autosamplers for repeat automated sample loading

NGC™ FRACTION COLLECTOR

IDEAL FOR HIGH-CAPACITY ANALYTICAL AND PREPARATIVE CHROMATOGRAPHY APPLICATIONS

Includes an NGC Fraction Collector.

  • Increased capacity to accommodate multiple racks for multi-vessel collection up to 200 ml/min
  • Collection flexibility with rack options: microtiter plates, centrifuge tubes, 15 and 50 ml conical tubes, and bottles
  • Benchtop Peltier cooling option to maintain integrity of fractions
  • Production batch capacity by collecting up to 80 unlimited volume fractions with prep-rack adaptor
  • Accurate vessel positioning down to millimeter precision using factory programed firmware
  • Integrated valve for diverting flow to waste

NGC FRACTION COLLECTOR WITH ONE 12-PORT OUTLET

IDEAL FOR HIGH-CAPACITY ANALYTICAL AND PREPARATIVE CHROMATOGRAPHY APPLICATIONS

Includes an NGC Fraction Collector and a 12-port outlet valve.

  • Increased capacity to accommodate multiple racks for multi-vessel collection up to 200 ml/min
  • Collection flexibility with rack options: microtiter plates, centrifuge tubes, 15 and 50 ml conical tubes, and bottles
  • Benchtop Peltier cooling option to maintain integrity of fractions
  • Production batch capacity by collecting up to 80 unlimited volume fractions with prep-rack adaptor
  • Accurate vessel positioning down to millimeter precision using factory programed firmware
  • Integrated valve for diverting flow to waste
  • Outlet valves for additional large-volume collection options

NGC FRACTION COLLECTOR WITH TWO 12-PORT OUTLETS

IDEAL FOR HIGH-CAPACITY ANALYTICAL AND PREPARATIVE CHROMATOGRAPHY APPLICATIONS

Includes an NGC Fraction Collector and two 12-port outlet valves.

  • Increased capacity to accommodate multiple racks for multi-vessel collection up to 200 ml/min
  • Collection flexibility with rack options: microtiter plates, centrifuge tubes, 15 and 50 ml conical tubes, and bottles
  • Benchtop Peltier cooling option to maintain integrity of fractions
  • Production batch capacity by collecting up to 80 unlimited volume fractions with prep-rack adaptor
  • Accurate vessel positioning down to millimeter precision using factory programed firmware
  • Integrated valve for diverting flow to waste
  • Outlet valves for additional large-volume collection options

BioFrac™ FRACTION COLLECTOR

IDEAL FOR ANALYTICAL AND PREPARATIVE CHROMATOGRAPHY APPLICATIONS

Includes a BioFrac fraction collector.

  • Basic or complex fraction collection schemes for flow rates of up to 100 ml/min.
  • Flexibility with numerous collection rack options such as microtiter plates, centrifuge tubes, 15 and 50 ml conical tubes, and bottles
  • Integrated valve for diverting flow to waste

BioFrac FRACTION COLLECTOR WITH ONE 12-PORT OUTLET

IDEAL FOR ANALYTICAL AND PREPARATIVE CHROMATOGRAPHY APPLICATIONS

Includes a BioFrac fraction collector and a 12-port outlet valve.

  • Basic or complex fraction collection for flow rates of up to 100 ml/min.
  • Flexibility with numerous collection rack options
  • Integrated valve for diverting flow to waste
  • Outlet valve for additional large volume collection options
  • Multicolumn purification for increased reproducibility and throughput

BioFrac FRACTION COLLECTOR WITH TWO 12-PORT OUTLETS

IDEAL FOR HIGH-CAPACITY ANALYTICAL AND PREPARATIVE CHROMATOGRAPHY APPLICATIONS

Includes a BioFrac fraction collector and two 12-port outlet valves.

  • Basic or complex fraction collection schemes for flow rates of up to 100 ml/min.
  • Flexibility with numerous collection rack options
  • Integrated valve for diverting flow to waste
  • Two outlet valves for maximum large volume collection options
  • Multicolumn purification for increased reproducibility and throughput

LARGE VOLUME COLLECTION — ONE 12-PORT OUTLET

IDEAL FOR PREPARATIVE CHROMATOGRAPHY APPLICATIONS

Includes a single 12-port outlet valve.

  • Outlet valve for additional large volume collection options
  • Multicolumn purification for increased reproducibility and throughput

LARGE VOLUME COLLECTION — TWO 12-PORT OUTLETS

IDEAL FOR PREPARATIVE CHROMATOGRAPHY APPLICATIONS

Includes two 12-port outlet valves.

  • Two outlet valves for maximum large volume collection options
  • Multicolumn purification for increased reproducibility and throughput

NO COLLECTION OR EXISTING COLLECTOR

FOR ANALYTICAL APPLICATIONS WHERE FRACTION COLLECTION IS NOT REQUIRED OR AN EXISTING COLLECTOR IS PRESENT

Dual Gradient Pump System...